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Product Details

IFN-g

Feldan Bio Inc
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Product Description:
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Recombinant Human Interferon-gamma
IFN-g Human

Catalog #: 1D-10-029

Clone:
IFN-g Human
Source:
E.coli

Description:
Interferon-gamma Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 16879 Dalton.
The IFN-gamma is purified by proprietary chromatographic techniques.

Synonyms:
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized after extensive dialysis from a concentrated (1mg/ml) solution in 10mM sodium Phosphate buffer pH=7.4±0.1.

Solubility:
It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Interferon gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.



Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Asp-Pro-Tyr.

Biological Activity:
The specific activity as determined in a viral resistance assay is less then 0.05 ng/ml, corresponding to a specific activity of 2.0 x 107 IU/ mg.

Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IFN-gamma as a Reference Standard.

Usage:
Feldan Bio's products are intended for LABORATORY RESEARCH USE ONLY.
Other Details:

Offered by: Feldan Bio Inc (View Showroom, Create your own Showroom)
Place of Origin: Canada (More Products from Canada)
Stock Location: Canada
Quantity: Not Specified
Packaging: Not Specified
Price: 75.00 (20µg) /180.00 (100µg) /1000.00 (1000µg) CAN
Product Seller Classification: Chemicals - Pharmaceutical Products
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